Human Heregulin beta-1 (AF)

Recombinant Neuregulin/Heregulin represents a group of closely related polypeptide growth factors generated from alternate gene splicing (NRG1, NRG2, NRG3, and NRG4). Currently, there are more than 14 soluble and membrane-bound proteins derived from the NRG1 gene. The extracellular segment of transmembrane NRG1 isoforms undergoes proteolytic cleavage, producing soluble growth factors. Notably, HRG1-?1 comprises an Immunoglobulin (Ig) domain alongside an Epidermal Growth Factor (EGF)-like domain; the latter is crucial for binding directly to the receptor tyrosine kinases erb3 and erb4. This binding event initiates erb3 and erb4 heterodimerization with erb2, thereby activating intrinsic kinase activity and leading to tyrosine phosphorylation. While the precise biological impacts of HRG1-?1 remain unclear, studies have shown its role in enhancing the movement and invasive properties of breast cancer cells, potentially involving the upregulation of autocrine motility-promoting factor (AMF) expression and functionality. Recombinant Human Heregulin?-1 (HRG1-B1) is a 7.5 kDa polypeptide composed solely of the EGF domain from heregulin?-1, spanning 65 amino acid residues.

Species	Human
Published species	Human, Mouse
Expression System	E. coli
Amino acid sequence	SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
Molecular weight	7.5 kDa
Class	Recombinant
Type	Protein
Purity	≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration	<0.1 EU/µg
Activity	The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ≤ 0.125 ng/ml, corresponding to a specific activity of ≥ 8 x 10^6 units/mg.
Conjugate	Unconjugated
Form	Lyophilized
Contains	no preservative
Storage conditions	-20°C

Code	Description	Pack Size
ZRP-HEREGB-10UG	Human Heregulin beta-1 (AF)	10ug
ZRP-HEREGB-50UG	Human Heregulin beta-1 (AF)	50ug
ZRP-HEREGB-100UG	Human Heregulin beta-1 (AF)	100ug
ZRP-HEREGB-500UG	Human Heregulin beta-1 (AF)	500ug
ZRP-HEREGB-1MG	Human Heregulin beta-1 (AF)	1mg

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