We are delighted to announce an exciting new partnership between TCS Biosciences and Takara Bio, bringing the renowned Cellartis® portfolio directly to researchers across the United Kingdom.
This collaboration strengthens our growing life science offering and provides UK scientists with easier access to Takara Bio's trusted stem cell technologies, supported by the dedicated service and technical expertise you expect from TCS Biosciences.
The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.
With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the system, coupled with its ability to ensure an efficient and predictable growth rate, makes the DEF-CS culture system ideal for the expansion and scale-up of a homogeneous population of iPS cells. Following reprogramming, successful expansion of your pluripotent stem cells is crucial. The DEF-CS culture system, a research-grade system, provides media, a coating reagent, and growth factors for the easy culture of iPS cells as a 2D monolayer.
Products Available
| Product Code | Description | Pack Size |
|---|---|---|
| Z-CEL-Y30010 | Cellartis® DEF-CS™ 500 Culture System | 1 Kit |
| Z-CEL-Y30012 | Cellartis® DEF-CS™ 500 COAT-1 | 4 mL |
| Z-CEL-Y30017 | Cellartis® DEF-CS™ 500 Basal Medium with Additives | 500 mL |
Overview
- Non-colony, 2D-monolayer format enables you to culture pluripotent stem cells just like any other cell type
- Feeder-free and chemically defined
- Cells maintain pluripotency and long-term potential for self-renewal and differentiation
- Robust system provides high reproducibility, stable growth, and ensures maintenance of a normal karyotype
- Complete kit includes 500 ml of basal medium, additives, and coating compound
Applications
- Single-cell culture of human iPS cells
- Transfection and reprogramming
- High-throughput screening
- Tissue engineering (seeding cells on a scaffold)
- Gene editing
References
Boreström, C. et al. Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source. Stem Cells Trans. Med. 3, 433–447 (2014).
Hanson, C. et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta ophthalmologica 91, 127–130 (2013).
Kia, R. et al. MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicol. Sci. 144, 173–85 (2015).
Norrman, K. et al. Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59, 59–70 (2013).
Sivertsson, L. et al. Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor. Stem Cells Dev. 22, 581–594 (2012).
Ulvestad, M. et al. Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem. Pharmacol. 86, 691–702 (2013).
Valton, J. et al. Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods 69, 151–170 (2014).
Zandén, C. et al. Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. NANOMED-NBM 10, 949–958 (2014).
Additional Information
Please refer to the product's Certificate of Analysis for detailed information on storage conditions, product components, and technical specifications. To view the contents of a kit, please consult the Kit Components List. Both documents can be found under the Documents tab on the Takara website, available here: https://www.takarabio.com/products/stem-cell-research
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