We are delighted to announce an exciting new partnership between TCS Biosciences and Takara Bio, bringing the renowned Cellartis® portfolio directly to researchers across the United Kingdom.
This collaboration strengthens our growing life science offering and provides UK scientists with easier access to Takara Bio's trusted stem cell technologies, supported by the dedicated service and technical expertise you expect from TCS Biosciences.
Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation System are an alternative to primary hepatocytes, as they exhibit sufficient expression levels of drug-metabolizing enzymes and transporters and demonstrate stable functionality over time in culture. In addition, hiPS cell-derived hepatocytes can provide an accurate reflection of the metabolic diversity observed in the human population.
The Cellartis iPS Cell to Hepatocyte Differentiation System provides a complete solution for generating functional, hiPS cell-derived hepatocytes within three weeks.
Overview
The process of generating hiPS cell-derived hepatocytes begins with the directed differentiation of hiPS cells into Definitive Endoderm (DE) cells that are then differentiated further into hepatocytes. The complete system provides media and ready-to-use coatings for each step of the iPS-cell-to-hepatocyte differentiation protocol. Starting with approximately 3 x 10⁶ undifferentiated hiPS cells, this system yields 5 x 10⁶ hepatocytes, equivalent to a confluent monolayer of 50 cm². This do-it-yourself system offers a solution for the consistent production of assay-ready cells from patient-derived cells or from Cellartis brand iPS cell lines—enabling highly reproducible results.
Hepatocytes obtained with the Cellartis iPS Cell to Hepatocyte Differentiation System express major hepatic markers and show relevant CYP enzyme activities based on the genetic background of the original hiPS cells from which they were derived. In addition, these freshly differentiated hepatocytes are functionally stable for a longer timeframe compared to cryopreserved primary hepatocytes, enabling prolonged experiments such as long-term toxicity assays or viral infection studies. Cellartis Hepatocyte Maintenance Medium (Cat. # Y30051) is recommended for long-term maintenance of hiPS cell-derived hepatocytes.
Product Components
| Product Code | Description | Notes |
|---|---|---|
| Z-CEL-Y30020 | Cellartis DEF-CS 100 Culture System | (not sold separately) |
| Z-CEL-Y30030 | Cellartis Definitive Endoderm Differentiation Kit | (not sold separately) |
| Z-CEL-Y30050 | Cellartis Hepatocyte Differentiation Kit | (not sold separately) |
Features
- Highly reproducible, robust system - the exact same protocol has been shown to work across 25 different iPS cell lines, so there's no need to optimize for your lines
- Ideal for drug metabolism and safety studies - consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds
- Customized starting materials - start with any disease-relevant iPS cell lines and create accurate liver disease models
- Ready for personalized medicine - make patient-specific, disease-specific cells for therapeutic applications
- Extended experimental window - hepatocytes can be used for a minimum of 11 days for functional tests
References
Please refer to the product's Certificate of Analysis for detailed information on storage conditions, product components, and technical specifications. To view the contents of a kit, please consult the Kit Components List. Both documents can be found under the Documents tab on the Takara website, available here: https://www.takarabio.com/products/stem-cell-research
Additional Information
Please refer to the product's Certificate of Analysis for detailed information on storage conditions, product components, and technical specifications. To view the contents of a kit, please consult the Kit Components List. Both documents can be found under the Documents tab on the Takara website, available here: https://www.takarabio.com/products/stem-cell-research
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