We are delighted to announce an exciting new partnership between TCS Biosciences and Takara Bio, bringing the renowned Cellartis® portfolio directly to researchers across the United Kingdom.
This collaboration strengthens our growing life science offering and provides UK scientists with easier access to Takara Bio's trusted stem cell technologies, supported by the dedicated service and technical expertise you expect from TCS Biosciences.
LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes. It has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE medium contains no proteins other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used xeno-free media. A phenol red-free GMP-grade version is also available.
Overview
Conventional culture systems typically require the addition of serum (or plasma) in order to achieve sufficient quantities of T cells for research applications, but the availability of autologous serum varies, and it has been demonstrated that T cells expanded in the absence of serum can offer improved performance (Janetzki et al. 2010; Medvec et al. 2018). By enabling robust expansion in the absence of human serum or plasma, LymphoONE medium is well-suited for immunotherapy research involving T cells.
T-cell activation using LymphoONE T-Cell Expansion Xeno-Free Medium in combination with anti-CD3 antibody, IL-2, and RetroNectin reagent results in enhanced T-cell expansion and yields higher proportions of naïve T cells relative to approaches employing IL-2 in combination with anti-CD3 and anti-CD28 antibodies.
As demonstrated by the data below, expansion in LymphoONE medium also yields higher proportions of naïve T cells as compared to other commonly used media, with or without the addition of serum.
Phenotypic analysis of T cells expanded using various culture media in the presence or absence of serum. Peripheral blood mononuclear cells (PBMCs) were cultured for four days under stimulation by anti-CD3 monoclonal antibody, IL-2, and RetroNectin reagent in CultiLife 215 Culture Bags with or without addition of human serum using LymphoONE T-Cell Expansion Xeno-Free Medium, X-VIVO 15 (Lonza), or AIM V (Thermo Fisher Scientific). The cells were then cultured at appropriate dilutions with or without serum for an additional six days in each of the respective media supplemented with IL-2. Human serum was added at a concentration of 1% from Day 0 to Day 7, and then at a concentration of 0.5% from Day 7 to Day 10. Expansion in LymphoONE medium resulted in higher proportions of cells expressing markers associated with a naïve T-cell phenotype (CD45RA+/CCR7+), with or without addition of serum.
LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes. The medium has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE medium contains no proteins other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used serum-free media. T-cell activation using LymphoONE T-Cell Expansion Xeno-Free Medium in combination with anti-CD3 antibody, IL-2, and RetroNectin reagent results in enhanced T-cell expansion and yields higher proportions of naïve T cells relative to approaches employing IL-2 in combination with anti-CD3 and anti-CD28 antibodies. LymphoONE medium also contains L-glutamine and streptomycin, and is confirmed to have low endotoxin levels (<0.3 EU/ml).
Product Available
| Product Code | Description | Pack Size |
|---|---|---|
| Z-CEL-WK552 | LymphoONE T-Cell Expansion Xeno-Free Medium | 10 * 1L bottles |
Features
- Xeno-free medium that enables efficient T-cell expansion with or without the addition of serum or plasma
- Supplemented with human serum albumin, recombinant human insulin, L-glutamine, and streptomycin
- Endotoxin tested (endotoxin <0.3 EU/ml)
- Compatible with CultiLife culture bags for large-scale expansion of T cells
- Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin reagent* enhances proliferation and leads to a higher proportion of naïve T cells
- Available as GMP grade medium for developing cell therapy applications
* T-cell expansion by RetroNectin stimulation for uses other than research requires a license from Takara Bio Inc.
Applications
- Expansion of T cells
- Gene transfer by lentiviral or retroviral transduction
- Enhance culture and expansion of T cells and CAR-T cells
References
Janetzki, S. et al. Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells. Cancer Immunol. Immunother. 59, 609–18 (2010).
Medvec, A. R. et al. Improved expansion and in vivo function of patient T cells by a serum-free medium. Mol. Ther. Methods Clin. Dev. 8, 65–74 (2018).
Additional Information
Please refer to the product's Certificate of Analysis for detailed information on storage conditions, product components, and technical specifications. To view the contents of a kit, please consult the Kit Components List. Both documents can be found under the Documents tab on the Takara website, available here: https://www.takarabio.com/products/stem-cell-research
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