Human BDNF (AF)

BDNF belongs to the NGF family of neurotrophic growth factors supporting neuron growth and survival. It binds to a low-affinity cell surface receptor LNGFR shared with other neurotrophins like NGF NT-3 and NT-4. However BDNF primarily exerts its neurotrophic effects through the high-affinity receptor gp145/trkB. Structurally BDNF is part of a 247 amino acid polypeptide precursor featuring an 18 amino acid signal sequence and a 110 amino acid propeptide. Recombinant Human/Murine/Rat BDNF exists as a 27.0 kDa homodimer comprising two 120 amino acid subunits held together by robust non-covalent interactions.

Species	Human, Mouse, Rat
Published species	Amphibian, Chicken, Human, Mouse, Non-human primate, Rat
Expression System	E. coli
Amino acid sequence	MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Molecular weight	27.0 kDa
Class	Recombinant
Type	Protein
Purity	≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration	<0.1 EU/µg
Activity	Bio-Assay using C6 cells. The ED50 was determined to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 10^5 units/mg.
Conjugate	Unconjugated
Form	Lyophilized
Contains	no preservative
Storage conditions	-20°C

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