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EliGene<sup>®</sup> Adenovirus RT Kit

EliGene® Adenovirus RT Kit

Pack Size: 50 reactions

Product Code: Z10-90036-RT

Compatible Instruments: Real-Time Systems 7000, 7300, 7500 Applied Biosystems, LightCycler® 480 and NANO fully compatible with RotorGene instruments.

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Note: Euro Equivalents shown for information only.

Clinical study and description and results:From a total of 350 clinical specimens analysed, 20 were positive for Adenovirus when tested with EliGene® Adenovirus RT kit and 330 were correctly analysed as negative. Sensitivity: 100%, Specificity: 100%.

Pathogen description: Adenoviruses represent the largest nonenveloped viruses. They are able to be transported through the endosome. They are about 70 to 90 nm in size, nonenveloped icosahedral viruses that are made from up to 252 capsomeres containing double-standard DNA. Infection is usually transmitted by droplets of respiratory or ocular secretions, by alimentary transfer or sexual intercourse and by the contact with contaminated water (the virus is able to live out of the body at temperature 20C and lower for a few weeks). The sites of the first reproduction are most often epithelial cells of the conjunctiva, nasopharynx and intestine. Adenovirus infection is clinically manifested by fever, upper respiratory tract infections, tonsillitis, laryngitis, bronchitis and pneumonia.

References: Bannister BA, Begg NT, Gillespie SH. 2000. Infectious Disease. Blackwell Science, 2th Ed. Jothikumar N, Cromeans TL, Hill VR, Lu X, Sobsey MD, Erdman DD. 2005. Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol. 71(6):3131-6. Hlinomazova Z, Loukotova V, Horackova M, Sery O. 2010. The treatment of HSV1 ocular infections using quantitative real-time PCR results. Acta Ophthalmol. 10:1755-3768.

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t: +44 (0)1296 714222
f: +44 (0)1296 714806

Botolph Claydon, Buckingham, MK18 2LR, United Kingdom

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TCS Biosciences Ltd
t: +44 (0)1296 714222  •  f: +44 (0)1296 714806  •  e:
Botolph Claydon, Buckingham, MK18 2LR, United Kingdom

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