Human PDGFbb (AF)

PDGFs are dimers linked by disulfide bonds composed of two 12.0-13.5 kDa polypeptide chains known as PDGF-A and PDGF-B. Among the three naturally occurring PDGFs (PDGF-AA PDGF-BB and PDGF-AB) they act as potent mitogens for various cell types such as smooth muscle cells connective tissue cells bone and cartilage cells and certain blood cells. These growth factors are stored in platelet α-granules and are released during platelet activation contributing to processes like hyperplasia chemotaxis embryonic neuron development and respiratory tubule epithelial cell development. PDGFs interact with two distinct signaling receptors: PDGFR-α a high-affinity receptor for all three PDGF forms and PDGFR-β which specifically interacts with PDGF-BB and PDGF-AB. Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer consisting of two β chains totaling 218 amino acids.

Species	Human
Published species	Hamster Human Mouse Rabbit Virus
Expression System	E. coli
Amino acid sequence	MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH
Molecular weight	17.5 kDa
Class	Recombinant
Type	Protein
Purity	≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration	<0.1 EU/µg
Activity	Determined by its ability to stimulate the proliferation of mouse 2E8 cells. The expected ED50 ≤ 0.5 ng/ml corresponding to a specific activity of ≥ 2 x 10^6 units/mg.
Conjugate	Unconjugated
Form	Lyophilised
Contains	no preservative
Storage conditions	-20°C

CAUTION

For Research Use Only. Not for use in diagnostic procedures.

Biomanufacturing

Research

Regenerative Medicine

Stem Cell Research

Tissue Engineering

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