Human TPO (AF)

TPO a growth factor specific to certain lineages emerges from the liver kidney and skeletal muscle. Its primary function involves stimulating the proliferation and maturation of megakaryocytes while fostering an increase in circulating platelet levels in vivo. TPO transmits its signals through the c-mpl receptor playing a crucial role as a regulator of circulating platelets. Both human and murine TPO display cross-species reactivity. The Human TPO gene encodes a 353 amino acid glycoprotein featuring a 21 amino acid signal sequence a 15 amino acid erythropoietin-like domain and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO comprising 174 amino acids forms a fully biologically active polypeptide weighing 18.6 kDa incorporating the erythropoietin-like domain from the full-length TPO protein.

Species	Human
Published species	Hamster, Human, Mouse, Rat
Expression System	E. coli
Amino acid sequence	SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
Molecular weight	18.6 kDa
Class	Recombinant
Type	Protein
Purity	≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration	<0.1 EU/µg
Activity	The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.
Conjugate	Unconjugated
Form	Lyophilised
Contains	no preservative
Storage conditions	-20°C

CAUTION

For Research Use Only. Not for use in diagnostic procedures.

Biomanufacturing

Research

Regenerative Medicine

Stem Cell Research

Tissue Engineering

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