Human Flt3-Ligand (AF)

Flt3-Ligand is a growth factor responsible for regulating the proliferation of early hematopoietic cells. It binds to cells expressing the Flt3 tyrosine kinase receptor. Interestingly Flt3-Ligand itself doesn't directly stimulate the proliferation of early hematopoietic cells. Instead it works synergistically with other colony-stimulating factors (CSFs) and interleukins to induce growth and differentiation. Unlike SCF Flt3-Ligand doesn't affect mast cells. Various isoforms of Flt3-Ligand have been identified with the predominant biologically active form being anchored to the cell surface as part of a transmembrane protein (209 amino acids). This membrane-bound isoform can undergo proteolytic cleavage producing a biologically active soluble isoform. Recombinant Human Flt3-Ligand is a soluble protein weighing 17.6 kDa and composed of 155 amino acid residues.

Species	Human
Published species	Hamster, Human, Mouse, Rabbit
Expression System	E. coli
Amino acid sequence	TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
Molecular weight	17.6 kDa
Class	Recombinant
Type	Protein
Purity	≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration	<0.1 EU/µg
Activity	The ED50 as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.
Conjugate	Unconjugated
Form	Lyophilised
Contains	no preservative
Storage conditions	-20°C

CAUTION

For Research Use Only. Not for use in diagnostic procedures.

Biomanufacturing

Research

Regenerative Medicine

Stem Cell Research

Tissue Engineering

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