Quality Control:
Standard product specification is a mean of 28-33 cfu with a standard deviation of less than 3. Also available is a 10,000 cfu BioBall™ which has a standard deviation of 20%.
Each batch of BioBall™ is quality controlled by testing a minimum of 10% of the batch up to a maximum of 50. Vials are selected at random; each standard batch of BioBall™ meets the following criteria:
| Standard Deviation |
<3 |
| Mean |
28-33 cfu |
Where the standard deviation is a measure of the batch variability and the mean is the average cfu count tested on non-selective agar.
Applications:
- Method validation
- Quantitative Quality Control
- Accreditation compliance
- Multi-laboratory standardisation
- Measurement of uncertainty
The BioBall™ strain list is constantly being updated. To ensure that you have the most up to date listing visit the BioBall product page or alternatively send an e-mail to bioball@tcsgroup.co.uk to join our Strain Alert System. We will email you details of new strains as they become available and issue an up-to-date product list.
BioBall™ MultiShot 550 is now available.
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Until recently, the manual immunomagnetic separation (IMS) procedure was the only step in the DWI approved Cryptosporidium method which had not been improved. TCS Water Sciences has now developed a semi automated IMS system, Isolate®, based on a kit developed by ImmuCell Corporation, USA.
Isolate® is a unique product offering semi automated immunomagnetic separation (IMS) of Cryptosporidium oocysts in environmental water samples. Isolate® is able to process pellets of up to 2 ml in volume, significantly reducing the need for split samples, and yields good recoveries even in dirty water samples. The automation of the
Isolate® test standardises the rocking motion, eliminating human error and achieving improved consistency and reliability of results.
Cryptosporidium oocysts in the concentrate are captured and separated by magnetic action. Separated oocysts are stained with an approved FITC antibody stain, and visualised by fluorescence microscopy.
TCS Water Sciences compliments the Isolate® Cryptosporidium test by offering a range of high quality Cryptosporidium testing products; our expertise in the field of Cryptosporidium enables us to offer you technical support and advice. TCS offers exceptional levels of customer service to ensure you get what you want when you want it.
The following table shows the results of a study which compared the Isolate® system with a manual IMS test. Isolate® achieved considerably
improved results with increased recovery of Cryptosporidium, and greater consistency, evidenced by the narrower data ranges. The results highlight the sensitivity of the manual method to operator technique and thus the superiority of automation.
|
Percentage recovery of Cryptosporidium from an upland treated water sample. |
| |
Manual IMS |
Isolate® |
| Packed Pellet |
0.5ml |
1ml |
0.5ml |
1ml |
| Mean (n = 10) |
49.7 |
48.4 |
83.7 |
80.4 |
| Range |
30-82 |
17-91 |
72-92 |
62-89 |
Percentage recovery of Cryptosporidium from a river water sample. |
| |
Manual IMS |
Isolate® |
| Packed Pellet |
0.5ml |
1ml |
0.5ml |
1ml |
| Mean (n = 10) |
54.2 |
30.0 |
68.1 |
75.4 |
| Range |
45-66 |
25-41 |
60-85 |
58-87 |
Materials supplied by TCS Water Sciences:
- Isolate® Cryptosporidium 100 Test Kit (ZAICS100)
- Isolate® Rotator Disc and Hub (ZAICS2)
- Incu-Clip (ZAICS3), Magna-Clip (ZAICS4), Micro-Clip (ZAICS5)
- Dissociation Block (ZAICS8)
- Variable speed rotator (ZAICSI)
- Glass Leighton tubes (ZAICS6)
Visit the Isolate® page
Download this article (pdf)
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New, from Cellabs, Cryptosporidium IFA 15 Minute stain.
High-definition monoclonal diagnostic test for rapid detection of Cryptosporidium oocysts in human faecal samples as well as water and sewage samples.
Cellabs are specialists in waterborne infections and are pleased to introduce this high performance diagnostic kit which enables the rapid detection of Cryptosporidium oocysts. The monoclonal antibody reacts specifically with the oocyst cell wall to give a clearly defined morphology, making accurate diagnosis possible.
No need for revalidation, DWI approved.
Download more information here (pdf)
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