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- IFA Reagent Against Cryptosporidium and Giardia
- 1gG1 Specificity
- 15 Minute Incubation
EasyStain™ an immunofluorescence reagent designed for use in testing water samples for the presence of Cryptosporidium oocysts and Giardia cysts. When a water sample is stained with the EasyStain™ reagent all oocysts or cysts present in the sample will fluoresce bright green. Stained samples can then be examined using fluorescence microscopy, flow cytometry or laser scanning cytometry.
The detection of oocysts and cysts in water is extremely difficult. There are many particles present in water samples that cross-react with Cryptosporidium and Giardia-specific antibodies. These particles can easily be mistaken for Cryptosporidium oocysts or Giardia cysts. EasyStain™ does not cross-react with particles present in water samples. This superior specificity is achieved by using monoclonal antibodies (mAbs) of the IgGl isotype.
EasyStain™ utilises a patented method for producing IgGl antibodies to Cryptosporidium and Giardia. These antibodies have: been demonstrated to be far superior to other available antibodies.
The antibodies in EasyStain™ have been purified to a very high level to ensure that there is no contamination. The conjugated antibody is stored in a specially designed buffer that includes a novel blocking agent and preservative.
EasyStain™ is a mixture of two monoclonal antibodies {mAb): a Cryptosporidium-specific IgGl mAb and a Giardia-specific IgGl mAb. The reagent is supplied as a working solution and does not require dilution.
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